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An optical fiber biosensor consisting of acetylcholinesterase (AChE) and bromothymol blue (BTB) doped sol-gel film was employed to detect organophosphate pesticide chlorpyrifos. The primary advantage of this optical biosensor is using a single sol-gel film with immobilized AChE and BTB. The compatibility of this mixture (AChE and BTB) with the sol-gel matrix has prevented leaching of the film. The immobilization of the enzyme and indicator was easy without chemical modification. The biosensing factor on single sol-gel film has been placed inside the circulation-cell for stream system. In the presence of a continuing AChE, a color change of the BTB and the measured mirrored signal at wavelength 622nm could possibly be associated to the pesticide concentration in the sample solutions. The performance of optical biosensor within the flow system has been optimized, together with chemical and physical parameters. The response time of the biosensor is 8min. A linear calibration curve of chlorpyrifos towards the proportion inhibition of AChE was obtained from 0.05 to 2.0mg/L of chlorpyrifos (18-80% inhibition, R(2)=0.9869, n=6). The detection limit for chlorpyrifos was 0.04mg/L. The outcomes of the analysis of 0.5-1.5mg/L of chlorpyrifos using this optical biosensor agreed nicely with chromatographic method.

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